IKK alpha (CHUK) pSer176/177 Rabbit Polyclonal Antibody

CAT#: AP55886PU-S

IKK alpha (CHUK) pSer176/177 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) Antibody.The lane on the right is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:500~1:1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK-alpha/beta (KLH-conjugated)
• Specificity: The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 85 kDa
• Gene Name: conserved helix-loop-helix ubiquitous kinase
• Database Link:
  Entrez Gene 1147 Human
  UniProt ID O15111
• Background: Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.
• Synonyms: CHUK, TCF16, I kappa-B kinase alpha, I kappa-B kinase beta, IkBKA, IkBKB, IKK-alpha, IKK-beta, IKK-A, IkappaB kinase, I-kappa-B kinase 1, NFKBIKA, IKK1, IKK2