SYK pTyr352 Rabbit Polyclonal Antibody

CAT#: AP55842PU-S

SYK pTyr352 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from HuvEc cells (Lane 2) and HepG2 cells (Lane 3), using SYK (Phospho-Tyr352) Antibody. The lane on the left is treated with antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:500~1:1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 352(S-P-Y(p)-A-D) derived from Human SYK (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 352.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 72 kDa
• Gene Name: spleen tyrosine kinase
• Database Link:
  Entrez Gene 6850 Human
  UniProt ID P43405
• Background: Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
• Synonyms: Tyrosine-protein kinase SYK, Spleen tyrosine kinase