GAB2 pSer623 Rabbit Polyclonal Antibody

CAT#: AP55821PU-S

GAB2 pSer623 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using Gab2 (Phospho-Ser623) Antibody. The lane on the left is treated with antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Gab2 (Phospho-Ser623) Antibody (left)or the same antibody preincubated with blocking peptide (right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin sections: 1:50~1:100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of Serine 623(P-S-S(p)-P-S) derived from Human Gab2 (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of Gab2 only when phosphorylated at serine 623.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 80 kDa
• Gene Name: GRB2 associated binding protein 2
• Database Link:
  Entrez Gene 9846 Human
  UniProt ID Q9UQC2
• Background: This gene is a member of the GRB2-associated binding protein (GAB) gene family and is similar to the GAB1 gene. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
• Synonyms: GRB2-associated-binding protein 2, KIAA0571, pp100