splicing factor 1 (SF1) pSer82 Rabbit Polyclonal Antibody

CAT#: AP55704PU-S

splicing factor 1 (SF1) pSer82 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from HUVEC cells treated with anisomycin using SF1 (Phospho-Ser82) Antibody.The lane on the right is treated with the antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody (left)or the same antibody preincubated with blocking peptide (right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin section: 1:50~1:100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1 (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 68 kDa
• Gene Name: splicing factor 1
• Database Link:
  Entrez Gene 7536 Human
  UniProt ID Q15637
• Background: Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
• Synonyms: ZFM1, ZNF162, BBP