PRKACA pThr197 Rabbit Polyclonal Antibody

CAT#: AP55702PU-N

PRKACA pThr197 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from mouse brain cells using PKA a/β CAT (Phospho-Thr197) Antibody.The lane on the right is treated with the antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA a/β CAT (Phospho-Thr197) antibody (left)or the same antibody preincubated with blocking peptide (right).

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  Immunofluorescence staining of methanol-fixed A549 cells using PKA a/β CAT (Phospho-Thr197) Antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin sections: 1:50~1:100.
  Immunofluorescence: 1:100~1:200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of threonine 197(R-T-W(p)-T-L) derived from Human PKA α/β CAT (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of PKA α/β CAT only when phosphorylated at threonine 197.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40 kDa
• Gene Name: protein kinase cAMP-activated catalytic subunit alpha
• Database Link:
  Entrez Gene 5566 Human
  UniProt ID P17612
• Background: Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
• Synonyms: PKA C-alpha / PKA C-alpha