Amyloid Precursor Protein (APP) pThr743/668 Rabbit Polyclonal Antibody

CAT#: AP55686PU-S

Amyloid Precursor Protein (APP) pThr743/668 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from cos-7 cells (Lane 2), using Amyloid β A4 (Phospho-Thr743/668) Antibody. The lane on the left is treated with antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human brain tissue using Amyloid β A4 (phospho-Thr743/668) antibody (left)or the same antibody preincubated with blocking peptide (right).

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  Immunofluorescence staining of methanol-fixed HeLa cells using Amyloid β A4 (phospho-Thr743/668) Antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin sections: 1:50~1:100.
  Immunofluorescence: 1:100~1:200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of threonine743 /668 (A-V-T(p)-P-E) derived from Human Amyloid β A4 (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of Amyloid β A4 only when phosphorylated at threonine 743/668.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 140 kDa
• Gene Name: amyloid beta precursor protein
• Database Link:
  Entrez Gene 351 Human
  UniProt ID P05067
• Background: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity By similarity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1.
• Synonyms: Alzheimer disease amyloid protein, Amyloid Precursor Protein, ABPP, APPI, PreA4, Cerebral vascular amyloid peptide, CVAP