Slc17a7 Rabbit Polyclonal Antibody

CAT#: AP55379PU-N

Slc17a7 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot using Immunopurifed VGLUT1 antibody

Specifications

Product Data

• Applications: ELISA, EM, IHC, WB
• Recommended Dilution: ELISA.
  RIA: 1/500
  Western Blot: 1/500.
  Electron Microscopy: 1/200.
  Immunohistochemistry.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A 17 residue synthetic peptide derived from C-terminal domain of Rat VGLUT1.
• Specificity: This antibody reacts with Rat 55-60 kDa VGLUT1 protein of BON cells stably expressing VGLUT1 and of total Rat brain extracts.
  No cross reactivity was detected with VGLUT2 and VGLUT3.
• Formulation: 0.1M Tris, 0.1 M Glycine, 2% Sucrose
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: solute carrier family 17 member 7
• Database Link:
  Entrez Gene 116638 Rat
  UniProt ID Q62634
• Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.
• Synonyms: Vesicular glutamate transporter 1, BNPI