COX2 (PTGS2) (Center) Rabbit Polyclonal Antibody

CAT#: AP53500PU-N

COX2 (PTGS2) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Cyclooxygenase 2 Antibody (Center) in Hela cell line lysates (35ug/lane). This demonstrates the PTGS2 antibody detected the PTGS2 protein (arrow).

• 

  Formalin fixed and paraffin embedded human uterus tissue stained with Cyclooxygenase 2 Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining

• 

  Confocal immunofluorescent analysis of Cyclooxygenase 2 Antibody (Center) with A549 cell followed by Alexa Fluor®489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 370-399 amino acids from the Central region of Human Cyclooxygenase 2.
• Specificity: This antibody recognizes Human Cyclooxygenase 2 (Center).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: prostaglandin-endoperoxide synthase 2
• Database Link:
  Entrez Gene 5743 Human
  UniProt ID P35354
• Background: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis
• Synonyms: Cyclooxygenase-2, PTGS2, COX2, PHS II, PGH synthase 2, PGHS-2, PGHS2
• Note: Molecular Weight: 68996 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway