Progesterone Receptor (PGR) (Center) Rabbit Polyclonal Antibody

CAT#: AP53273PU-N

Progesterone Receptor (PGR) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PGR Antibody (Center) in T47D cell line lysates (35ug/lane). This demonstrates the PGR antibody detected the PGR/PR protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue reacted with PGR Antibody (Center), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PGR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 348-377 amino acids from the Central region of human Progesterone receptor
• Specificity: This antibody recognizes Human Progesterone receptor (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: progesterone receptor
• Database Link:
  Entrez Gene 5241 Human
  UniProt ID P06401
• Background: This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform A has not been clearly determined.
• Synonyms: PR, PGR, NR3C3
• Note: Molecular Weight: 98981 Da

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Oocyte meiosis, Progesterone-mediated oocyte maturation