Osteopontin (SPP1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP52999PU-N

Osteopontin (SPP1) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of OPN-a/b (arrow) using rabbit polyclonal OPN-a/b Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OPN-a/b gene.

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with OPN-a/b Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of OPN-a/b Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1:1000
  Immunofluorescence: 1:25
  Immunohistochemistry on Paraffin Sections: 1:50~100
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 14-40 amino acids from the N-terminal region of Human Osteopontin (OPN-a/b)
• Specificity: This antibody recognizes Human Osteopontin (OPN-a/b) (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: secreted phosphoprotein 1
• Database Link:
  Entrez Gene 6696 Human
  UniProt ID P10451
• Background: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).
• Synonyms: BNSP, OPN, SPP-1, Nephropontin, Uropontin
• Note: Molecular Weight: 35423 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway