Neutrophil Elastase (ELANE) (N-term) Rabbit Polyclonal Antibody

CAT#: AP52858PU-N

Neutrophil Elastase (ELANE) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in A2058 cell line lysates (35ug/lane) using Neutrophil elastase antibody. (N-term). This demonstrates this antibody detected the Neutrophil elastase protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution:

  Western blot: 1/1000.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - between 13-39 amino acids from the N-terminal region (between 20-47aa ) of human Neutrophil elastase
• Specificity: This antibody recognizes human Neutrophil elastase at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: elastase, neutrophil expressed
• Database Link:
  Entrez Gene 1991 Human
  UniProt ID P08246
• Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
• Synonyms: ELANE, ELA2, Neutrophil elastase, Elastase-2, PMN elastase, Bone marrow serine protease, Medullasin, Human leukocyte elastase, HLE
• Note: Molecular Weight: 28518 Da

Reference Data

• Protein Families: Protease, Transmembrane
• Protein Pathways: Systemic lupus erythematosus