MTX2 (C-term) Rabbit Polyclonal Antibody

CAT#: AP52772PU-N

MTX2 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane) using Metaxin-2 antibody. (C-term). This demonstrates this antibody detected the Metaxin-2protein (arrow).

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  Immunohistochemistry analysis in human skeletal muscle (Formalin-fixed, Paraffin-embedded) using Metaxin-2 antibody. (C-term), followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram) using Metaxin-2 antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 235-265aa) of human Metaxin-2.
• Specificity: This antibody recognizes Metaxin-2 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: metaxin 2
• Database Link:
  Entrez Gene 10651 Human
  UniProt ID O75431
• Background: The protein encoded by the MTX2 gene is highly similar to the metaxin 2 protein from mouse, which has been shown to interact with the mitochondrial membrane protein metaxin 1. Because of this similarity, it is thought that the encoded protein is peripherally associated with the cytosolic face of the outer mitochondrial membrane, and that it is involved in the import of proteins into the mitochondrion. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 7.
• Synonyms: MTX2
• Note: Molecular Weight: 29763 Da