MED25 (N-term) Rabbit Polyclonal Antibody

CAT#: AP52653PU-N

MED25 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MED25 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). This demonstrates the MED25 antibody detected the MED25 protein (arrow).

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  Western blot analysis of MED25 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). This demonstrates the MED25 antibody detected the MED25 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with MED25 Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 119-148 amino acids from the N-terminal region of human MED25.
• Specificity: This antibody recognizes Human and Mouse MED25 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: mediator complex subunit 25
• Database Link:
  Entrez Gene 75613 MouseEntrez Gene 81857 Human
  UniProt ID Q71SY5
• Background:
  This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].
• Synonyms: ACID1, ARC92, PTOV2, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78
• Note: Molecular Weight: 78171 Da