MAF1 (Center) Rabbit Polyclonal Antibody

CAT#: AP52581PU-N

MAF1 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MAF1 Antibody (Center) in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the MAF1 antibody detected the MAF1 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma reacted with MAF1 Antibody (Center), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

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  Flow cytometric analysis of Neuro-2a cells using MAF1 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 97-125 amino acids from the Central region of human MAF1
• Specificity: This antibody recognizes Human and Mouse MAF1 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: MAF1 homolog, negative regulator of RNA polymerase III
• Database Link:
  Entrez Gene 84232 Human
  UniProt ID Q9H063
• Background: This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.
• Synonyms: Repressor of RNA polymerase III transcription MAF1 homolog
• Note: Molecular Weight: 28771 Da

Reference Data

• Protein Families: Transcription Factors