Haptoglobin (HP) (Center) Rabbit Polyclonal Antibody

CAT#: AP52089PU-N

Haptoglobin (HP) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HP (arrow) using Haptoglobin Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene.

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  Western blot analysis of Haptoglobin Antibody (Center) in MDA-MB453, CEM, NCI-H460, HepG2, K562 cell line lysates (35ug/lane). This demonstrates the HP antibody detected the HP protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma reacted with Haptoglobin Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of Haptoglobin Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of MDA-MB435 cells using Haptoglobin Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: Western Blot: ~ 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 296-322 amino acids from the Central region of Human Haptoglobin
• Specificity: This antibody recognizes Human Haptoglobin (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: haptoglobin
• Database Link:
  Entrez Gene 3240 Human
  UniProt ID P00738
• Background: This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: BP; haptoglobin; HP2-ALPHA-2; HPA1S; MGC111141
• Note: Molecular Weight: 45205 Da

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane