Melatonin Related Receptor (GPR50) (Center) Rabbit Polyclonal Antibody

CAT#: AP51929PU-N

Melatonin Related Receptor (GPR50) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GPR50 (arrow) in K562 cell line lysates (35ug/lane) using GPR50 antibody.

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  Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) using GPR50 antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the center region (between 323-352aa) of human GPR50.
• Specificity: This antibody recognizes GPR50.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: G protein-coupled receptor 50
• Database Link:
  Entrez Gene 9248 Human
  UniProt ID Q13585
• Background: Melatonin-related receptor expression has been documented from human CNS tissues, specifically in hypothalamus and pituitary, but not in human peripheral tissues. In animals, expression has also been seen in the CNS, and peripheral tissues.
• Synonyms: Melatonin-related receptor, G protein-coupled receptor 50, H9
• Note: Molecular Weight: 67355 Da

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction