Glucose 6 phosphate isomerase (GPI) (C-term) Rabbit Polyclonal Antibody

CAT#: AP51910PU-N

Glucose 6 phosphate isomerase (GPI) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

GPI Antibody (C-term) western blot analysis in A2058, Y79, Ramos, A375, K562 and mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the GPI antibody detected the GPI protein (arrow).

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  Western blot analysis of GPI Antibody (C-term) in mouse Neuro-2a cell lysates (35ug/lane).GPI (arrow) was detected using the purified Pab.

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  GPI Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 452~481 amino acids from the C-terminal region of human GPI
• Specificity: This antibody reacts to GPI.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 63147 Da
• Gene Name: glucose-6-phosphate isomerase
• Database Link:
  Entrez Gene 2821 Human
  UniProt ID P06744
• Background: GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
• Synonyms: Phosphoglucose isomerase, PGI, Phosphohexose isomerase, PHI, Neuroleukin, NLK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism