GNAS (C-term) Rabbit Polyclonal Antibody

CAT#: AP51874PU-N

GNAS (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GNAS (arrow) using GNAS Antibody (C-term) Cat.-No AP51874PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene.

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  Western blot analysis of GNAS Antibody (C-term) Cat.-NoAP51874PU-Nin 293, NCI-H292 cell line lysates (35ug/lane). This demonstrates the GNAS antibody detected the GNAS protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue reacted with GNAS Antibody (C-term) Cat.-NoAP51874PU-Nfollowed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of GNAS Antibody (C-term) Cat.-NoAP51874PU-Nwith 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of 293 cells using GNAS Antibody (C-term) Cat.-NoAP51874PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of Human GNAS
• Specificity: This antibody recognizes Human GNAS (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: GNAS complex locus
• Database Link:
  Entrez Gene 2778 Human
  UniProt ID Q5FWY2
• Background: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
• Synonyms: AHO; C20orf45; GNAS1; GNASXL; GPSA; GSA; GSP; MGC33735; NESP; NESP55; OTTHUMP00000031742; OTTHUMP00000196026; OTTHUMP00000196030; PHP1A; PHP1B; POH; SCG6; XLalphas
• Note: Molecular Weight: 44;250 Da