GCNT3 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51804PU-N

GCNT3 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GCNT3 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). This demonstrates the GCNT3 antibody detected the GCNT3 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma reacted with GCNT3 Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of NIH-3T3 cells using GCNT3 Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 390-418 amino acids from the C-terminal region of Human GCNT3
• Specificity: This antibody recognizes Human and Mouse GCNT3 (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: glucosaminyl (N-acetyl) transferase 3, mucin type
• Database Link:
  Entrez Gene 72077 MouseEntrez Gene 9245 Human
  UniProt ID O95395
• Background: This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.
• Synonyms: C2GnT-mucin type, C2/4GnT
• Note: Molecular Weight: 50864 Da

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis