POC1B-GALNT4 (N-term) Rabbit Polyclonal Antibody

CAT#: AP51769PU-N

POC1B-GALNT4 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GALNT4 Antibody (N-term) in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).

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  Flow cytometric analysis of Hela cells using GALNT4 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 100-128 amino acids from the N-terminal region of Human GALNT4
• Specificity: This antibody recognizes Human GALNT4 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: POC1B-GALNT4 readthrough
• Database Link:
  Entrez Gene 8693 HumanEntrez Gene 100528030 Human
  UniProt ID Q8N4A0
• Background: This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon.
• Synonyms: GalNAc transferase 4, pp-GaNTase 4, GalNAc-T4
• Note: Molecular Weight: 66608 Da

Reference Data

• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis