DNMT3L (C-term) Rabbit Polyclonal Antibody

CAT#: AP51304PU-N

DNMT3L (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of DNMT3L Antibody (C-term) in Jurkat cell line lysates (35ug/lane). This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 354-381 amino acids from the C-terminal region of Human DNMT3L
• Specificity: This antibody recognizes Human DNMT3L (C-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: DNA (cytosine-5-)-methyltransferase 3-like
• Database Link:
  Entrez Gene 29947 Human
  UniProt ID Q9UJW3
• Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined.
• Synonyms: MGC1090
• Note: Molecular Weight: 43670 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways