CHST3 (N-term) Rabbit Polyclonal Antibody

CAT#: AP50912PU-N

CHST3 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in mouse spleen tissue lysates (35ug/lane) using CHST3 antibody. (N-term). This demonstrates the CHST3 antibody detected the CHST3 protein (arrow).

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  Immunohistochemistry analysis in human skeletal muscle using CHST3 antibody., followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHST3 antibody for IHC; Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 32-62aa) of human CHST3.
• Specificity: This antibody recognizes CHST3 at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: carbohydrate sulfotransferase 3
• Database Link:
  Entrez Gene 9469 Human
  UniProt ID Q7LGC8
• Background: CHST3 is an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation.
• Synonyms: Carbohydrate sulfotransferase 3, Chondroitin 6-sulfotransferase, C6ST-1, C6ST1, GST-0
• Note: Molecular Weight: 54706 Da

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis