ATP6V1A (Center) Rabbit Polyclonal Antibody

CAT#: AP50305PU-N

ATP6V1A (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ATP6V1A Antibody (Center) in A2058 cell line lysates (35ug/lane). ATP6V1A (arrow) was detected using the purified Pab.

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  ATP6V1A Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP6V1A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 447~477 amino acids from the Central region of human ATP6V1A
• Specificity: This antibody reacts to ATP6V1A.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ATPase H+ transporting V1 subunit A
• Database Link:
  Entrez Gene 523 Human
  UniProt ID P38606
• Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues.
• Synonyms: V-ATPase subunit A, ATP6A1, ATP6V1A1, VPP2
• Note: Molecular Weight: 68304 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection