PSMC3 Rabbit Polyclonal Antibody

CAT#: AP42156PU-N

PSMC3 rabbit polyclonal antibody, Aff - Purified

Product Images

Human HepG2; WB Suggested Anti-PSMC3 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; PSMC3 antibody - middle region (AP42156PU-N) in Human HepG2 cells using Western Blot

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  Human kidney; PSMC3 antibody - middle region (AP42156PU-N) in Human kidney cells using Immunohistochemistry

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blotting (1 - 3 µg/ml)
  Immunohistochemsitry on paraffin embedded sections (4 - 8 µg/ml)
• Reactivities: Bovine, Canine, Chicken, Human, Mouse, Rat, Zebrafish, African clawed frog
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-PSMC3 antibody: synthetic peptide directed towards the middle region of human PSMC3.
• Formulation: State: Aff - Purified
  State: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Purification: Purified using Protein A affinity column.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: proteasome 26S subunit, ATPase 3
• Database Link:
  Entrez Gene 19182 MouseEntrez Gene 29677 RatEntrez Gene 5702 Human
  UniProt ID P17980
• Background: PSMC3 is a subunit of the 26S proteasome. 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.
• Synonyms: 26S protease regulatory subunit 6A, Proteasome 26S subunit ATPase 3, Proteasome subunit P50, Tat-binding protein 1, TBP-1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Proteasome