p15 INK4b (CDKN2B) (Isoform 2) (C-term) Goat Polyclonal Antibody

CAT#: AP32957PU-N

p15 INK4b (CDKN2B) (Isoform 2) (C-term) goat polyclonal antibody, Aff - Purified

Product Images

(1 ug/ml) staining of Human Gastrointestinal cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 1-3 μg/ml. Approx 12+14kDa bands observed in Human Gastrointestinal cancer lysates (calculated MW of 8.1kDa according to NP_511042.1). The observed 14kDa cannot represent isoform 1 and therefore it most likely represents another posttranslational modification of isoform 2.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_004927.2; NP_511042.1.
• Specificity: This antibody is expected to recognize Isoform 2 (NP_511042.1) only.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cyclin-dependent kinase inhibitor 2B
• Database Link:
  Entrez Gene 1030 Human
  UniProt ID P42772
• Synonyms: p14INK4b, MTS2, p14-INK4b, p15-INK4b