GAP43 (C-term) Chicken Polyclonal Antibody

CAT#: AP32844PU-N

GAP43 (C-term) chicken polyclonal antibody, Aff - Purified

Product Images

Grified from the neonatal Mouse brain (green satining). Red staining corresponds to a Rabbit anti-Alpha III Spectrin. Blue staining corresponds to the Hoechst nuclear satin.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Quality Control: Quality assurance analysis was performed using Immunohistochemistry (at 1/1000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/1000 dilution) as a secondary antibody.
  Recommended Dilutions: 1/1000-1/2000 for Immunohistochemstry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells and 1/2000-1/5000 for western blots.
• Reactivities: Mouse
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized.
• Specificity: Specific for the ~43k GAP-43 protein.
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified IgY fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: growth associated protein 43
• Database Link:
  UniProt ID P17677
• Background: GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).
• Synonyms: Neuromodulin