APPL (APPL1) (C-term) Goat Polyclonal Antibody

CAT#: AP32796PU-N

APPL (APPL1) (C-term) goat polyclonal antibody, Aff - Purified

Product Images

AP32796PU-N (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/2000 (Detection Limit).
  Western blot: 0.3-1.0 μg/ml. Detects a band of Approx 90kDa in Human Heart lysates (calculated MW of 79.7kDa according to NP_036228.1).
  A customer has reported that this antibody also works well in Mouse (hepatocytes, C2C12 myoblasts and Mouse embryonic fibroblast cells).
• Reactivities: Human, Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_036228.1.
• Specificity: Recognizes Human APPL / DIP13 alpha (C-term).
  Other species not tested.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
• Database Link:
  Entrez Gene 26060 Human
  UniProt ID Q9UKG1
• Background: The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
• Synonyms: Dip13-alpha, KIAA1428