IGFBP1 (156-170) Goat Polyclonal Antibody

CAT#: AP32344PU-N

IGFBP1 (156-170) goat polyclonal antibody, Aff - Purified

Product Images

Staining of Human Placenta lysate using IGFBP1 Antibody at 2 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/64000 (Detection Limit).
  Western blot: 1-3 μg/ml. Detects a band of Approx 30kDa band observed in Human Placenta lysates (calculated MW of 27.9kDa according to NP_000587.1).
  An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
  Immunohistochemistry on Paraffin Sections: 10µg/ml in paraffin embedded Human Testis.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of Human IGFBP1 (NP_000587.1)
• Specificity: This antibody recogniztes Human IGFBP1 (156-170).
  Other species not tested.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: insulin like growth factor binding protein 1
• Database Link:
  Entrez Gene 3484 Human
  UniProt ID P08833
• Synonyms: IGF-binding protein 1, IGFBP-1, IBP1, IBP-1, Placental protein 12, PP12