Synaptotagmin 1 (SYT1) Chicken Polyclonal Antibody

CAT#: AP31821PU-N

Synaptotagmin 1 (SYT1) chicken polyclonal antibody, Aff - Purified

Product Images

Western blot showing a single Synaptotagmin bond running at about 65 kDa

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions:
  1/2000-1/5000 for Western Blots.
  1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control: Each of the four antibodies was analyzed by Immunohistochemistry (at a dilution of 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Human, Mouse
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Four different antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_033332) and Human (NP_005630) gene products.
  Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.4 mg/ml. Finally, equal volumes of each of the four affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
  Antibodies were affinity-purified and mixed in equal concentrations.
• Specificity: Recognizes Synaptotagmin-1.
• Formulation: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using a peptide column.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted in the dark at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: synaptotagmin 1
• Database Link:
  Entrez Gene 20979 MouseEntrez Gene 6857 Human
  UniProt ID P21579
• Background: Human Synaptotagmin-1 is a 47,442 dalton protein (422 amino acids) associated with pre-synaptic terminals of the PNS and CNS. Synaptotagmin-1 binds calcium and initiates the fusion of vesicles with the pre-synaptic membrane. This protein is also believed to be the target of proteolytic activity associated with Botulinum neurotoxin A.
• Synonyms: SYT1, SVP65, SYT, Synaptotagmin1, Synaptotagmin I, SytI, p65

Reference Data

• Protein Families: Secreted Protein, Transmembrane