Histone H3 Sheep Polyclonal Antibody

CAT#: AP31733PU-N

Histone H3 sheep polyclonal antibody, Purified

Product Images

Immunohistochemical analysis using Histone H3 antibody Cat.-No AP31733PU-N at 5 g/ml on Human Prostate tissue (Formalin-fixed, Paraffin-embedded).

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunocytochemistry: 1/100.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot: 1/300.
• Reactivities: Bovine, Human
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Intact Calf Histone H3 at 15.4 kD, 136 amino acids, complexed with RNA.
• Specificity: Recognizes Histone H3.
  Minimal cross-reactivity with linker Histone H1 and no cross-reactivity with Histone core proteins H2A, H2B, or H4.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
• Synonyms: H3 Histone family, HIST1H3