Red Blood Cells Rabbit Polyclonal Antibody

CAT#: AP31657PU-N

Red Blood Cells rabbit polyclonal antibody, Ig Fraction

Specifications

Product Data

• Applications: CT
• Recommended Dilution: Cytotoxic assays.
  Hemagglutination:
  Test Cell Suspension: 100 μl of washed fixed mouse 1% red blood cells in saline
  Product Tested: Diluted antibody in 100 μl normal rabbit serum diluted 1/100 in saline.
  Incubation: 37°C for 20 mins, in U-bottom microtiter plates
  20°C for 2 to 4 hrs. for cell settling pattern
  Titer: The highest dilution of the product that yields agglutination.
  Results: Titer 1/3200
  Ouchterlony:
  Sample Size: 10 μl
  Distance between wells: 5 mm
  Test time: 24 hours
  Antiserum tested: Goat anti-rabbit IgG
  Results: Positive
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Specificity: This antibody reacts to Red Blood Cell.
• Formulation: PBS
  State: Ig Fraction
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Salt fractionation and DEAE ion exchange chromatography, followed by 0.2 µm filtration.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Synonyms: RBCs, RBC