Alb Rabbit Polyclonal Antibody
Alb rabbit polyclonal antibody, Azide Free
Product Datasheet for 'AP31514AF-N'
|Applications||ELISA, ID, IF, IHC, WB|
|Recommend Dilution||The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
|Immunogen||Highly purified albumin isolated from Rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
|Specificity||The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results:
++ Mouse, Guinea Pig.
± Monkey, swine, Horse, Human.
- Sheep, Rabbit, Goat, Canine, Chicken, Bovine.
|Formulation||PBS, pH 7.2 without preservatives,as it may interfere with the antibody activity. No foreign proteins added.
State: Azide Free
State: Lyophilised hyperimmune purified IgG fraction
|Reconstitution Method||Restore with 1 ml sterile distilled water|
|Purification||Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation
and purification of the IgG fraction by DEAE-chromatography.
|Gene Name||Rattus norvegicus albumin (Alb)|
|Synonyms||ALB, BSA, HSA, Serum Albumin|
|Note||Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.|
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