Alb Rabbit Polyclonal Antibody

CAT#: AP31512FC-N

Alb rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: ID, IF, R
• Recommended Dilution: Can be used as reagent for the direct detection of Albumin in Mouse cells, tissues and body fluids in Immunofluorescence techniques.
  This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended Dilutions: 1/20-1/80.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Albumin is isolated from Mouse serum by Sequential Precipitation and purified by Ion Exchange Chromatography and Affinity Chromatography.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The defined antibody specificity is directed to Albumin as tested against Mouse sera. In Immunoelectrophoresis and double radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against Mouse serum and the purified Albumin.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement.
  This antiserum has been tested for cross-reactivity in Double Radial Immunodiffusion with the following results:
  Bovine (-), Chicken (-), Canine (-), Goat (-), Guinea Pig (++), Hamster (+), Horse (+), Human (+), Monkey (+), Rat (++), Sheep (-), Swine (+).
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: FITC
  State: Lyophilized purified hyperimmune IgG fraction
  Label: Fluorescein isothiocyanate isomer 1
  Absorption emission: 492 nm / 515 nm
  Molar radio: Fluorescein/IgG ~1.7
• Reconstitution Method: Restore by adding 1 ml of sterile distilled water
• Concentration: lot specific
• Purification: Hyperimmune with strong precipitating activity are selected for fractionation by Salt Precipitation and the purification of the IgG fraction by DEAE Chromatography
• Conjugation: FITC
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: albumin
• Database Link:
  Entrez Gene 11657 Mouse
  UniProt ID P07724
• Background: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
• Synonyms: ALB, BSA, HSA, Serum Albumin
• Note: Conjugation procedure: A proprietary technique for the binding to FITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.