Prostatic Acid Phosphatase (ACPP) (269-282) Goat Polyclonal Antibody

CAT#: AP31101PU-N

Prostatic Acid Phosphatase (ACPP) (269-282) goat polyclonal antibody, Aff - Purified

Product Images

ACPP antibody staining of Human Prostate lysate at 0.01 ug/ml (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  ACPP antibody staining of paraffin embedded Human Prostate at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/32000.
  Western Blot: 0.01-0.03 µg/ml. Approx 50kDa band observed in Human Prostate lysates.
  Immunohistochemistry on Paraffin Sections: 3-5 μg/ml. In paraffin embedded Human Prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001127666.1, NP_001090.2.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001127666.1; NP_001090.2).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: acid phosphatase, prostate
• Database Link:
  Entrez Gene 55 Human
  UniProt ID P15309
• Background: Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.
• Synonyms: Prostate acid phosphatase, PAP, ACP3, PSAP
• Note: Calculated Molecular Weight: 48.3kDa (NP_001127666.1).

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Riboflavin metabolism