Gap43 (C-term) Chicken Polyclonal Antibody

CAT#: AP26435SU-N

Gap43 (C-term) chicken polyclonal antibody, Ig Fraction

Product Images

Western blot of rat cortex lysate showing specific immunolabeli ng of the ~ 43k GAP43 protein.

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  Immunochemical staining of mixed neuron/glial cultures showing GAP43 (green) labeling of numerous axonal and dendritic profiles and anti-alpha II spectrin (red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/2000.
  Immunoflourescence: 1/500.
• Reactivities: Human, Mouse, Rat
• Host: Chicken
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region of the rat GAP43 sequence
• Specificity: This antibody detects GAP43.
• Formulation: PBS
  State: Ig Fraction
  State: Liquid IgY fraction
  Preservative: 10 mM NaN3
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: growth associated protein 43
• Database Link:
  Entrez Gene 29423 Rat
  UniProt ID P07936
• Background: GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser 41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).
• Synonyms: Neuromodulin