BCL2 pThr56 Rabbit Polyclonal Antibody

CAT#: AP26416AF-N

BCL2 pThr56 rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: ELISA: Reacts with synthetic peptide (SQPGHTpPHPASR) coated plate.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from human Bcl-2, conjugated with KLH for immunization
• Specificity: This antibody reacts with Human and other species with consensus Bcl-2 sequence SQPGHTpPHPASR.
• Formulation: 0.01M PBS, pH 7.4
  State: Azide Free
  State: Lyophilized Ig fraction
• Reconstitution Method: Restore with water to adjust the final concentration to 1.00 mg/ml.
• Purification: Protein G affinity
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 month or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: B-cell CLL/lymphoma 2
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Background: Bcl-2 is a 25kD inner mitochondrial membrane protein with anti-apoptotic function. It is believed that Bcl-2 suppresses apoptosis by inhibiting mitochondrial cytochrome C release, blocking caspase cascade and regulating mitochondrial calcium homeostasis. Bcl- 2 is identified from the study of B-cell lymphoma. About 90% of follicular lymphoma is caused by Bcl-2 overexpression resulted from the translocation of Bcl-2 gene from chromosome 18 to the immunoglobulin heavy chain enhancer on chromosome 14. Bcl-2 is also involved with other malignancies such as melanoma, breast cancer, prostate cancer and lung cancer. Bcl-2 Thr56 phosphorylation by p38 MAPK results in cytochrome C release. Mutation near Thr56 aborted Bcl-2 antiapoptotic function.
• Synonyms: BCL2, Bcl-2 alpha