TNF alpha (TNF) Rabbit Polyclonal Antibody

CAT#: AP26364PU-N

TNF alpha (TNF) Rabbit Polyclonal Antibody

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Immunohistochemsitry on Frozen Sections: The typical starting working dilution is 1/10.
  Immunohistochemistry on Paraffin Sections: The typical starting working dilution is 1/10.
  Flow Cytometry: The typical starting working dilution is 1/10.
  Functional Assays (inhibition of biological activity).
  Immunoassays.
  Western blot: The typical starting working dilution is 1/10.
• Reactivities: Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Specificity: The antibody reacts with natural and recombinant TNF-alpha and recognizes membrane and receptor bound TNF-alpha.
  The antibody shows Neutralizing activity.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 21926 Mouse
  UniProt ID P06804
• Background:

  
  Tumor Necrosis Factor alpha (TNF-alpha) is a cytokine which has diverse immunomodulatory, anti-tumor and toxic effects. TNF-alpha has been detected in diverse inflammatory status and appears to be a critical mediator in the lethality of septic shock. Furthermore, TNF-alpha has also been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants.

• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin