Foxp3 (C-term) Goat Polyclonal Antibody

CAT#: AP23767PU-N

Foxp3 (C-term) goat polyclonal antibody, Aff - Purified

Product Images

FOXP3 antibody staining of Paraffin Embedded Mouse Thymus at 2 ug/ml (left panel) of paraffin embedded Mouse Thymus. Right panel shows staining without primary antibody as negative control. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: 1/4000.
  Immunohistochemistry: 2-4 µg/ml. In paraffin embedded Mouse Thymus shows nuclear staining in some patches of thymocytes.
• Reactivities: Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_473380.1.
• Specificity: Recognizes FOXP3 / Scurfin (Mouse).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: forkhead box P3
• Database Link:
  Entrez Gene 20371 Mouse
  UniProt ID Q99JB6
• Background: FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.
  FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
• Synonyms: Forkhead box protein P3, IPEX, JM2, Scurfin