Lgals1 (N-term) Goat Polyclonal Antibody

CAT#: AP23675PU-N

Lgals1 (N-term) goat polyclonal antibody, Aff - Purified

Product Images

Galectin-1 antibody staining of Mouse (A) and Rat (B) Pancreas lysates and NIH3T3 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/8000.
  Western Blot: 0.1-0.3 µg/ml. Approx 15kDa band observed in lysates of Mouse and Rat Pancreas and of cell line NIH3T3.
• Reactivities: Bovine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the N-Terminus (near) of the protein sequence according to NP_032521.1.
• Specificity: Recognizes Mouse Galectin-1 (N-term).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: lectin, galactose binding, soluble 1
• Database Link:
  Entrez Gene 16852 Mouse
  UniProt ID P16045
• Background: Galectin-1 is a member of the beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This protein is an autocrine negative growth factor that regulates cell proliferation. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes, thus Galectin-1 has immunosuppressive and anti-inflammatory properties. Galectin-1 also regulates tumor angiogenesis and is a target for angiostatic cancer therapy.
• Synonyms: LGALS1, Galectin1, Lectin galactoside-binding soluble 1, Beta-galactoside-binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1, Galaptin, 14 kDa lectin, HPL, HBL, MAPK-activating protein PM12
• Note: Calculated Molecular Weight: 14.9kDa (NP_032521.1).