IRAK4 (2-13) Goat Polyclonal Antibody

CAT#: AP23599PU-N

IRAK4 (2-13) goat polyclonal antibody, Aff - Purified

Product Images

(1ug/ml) Staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/32000.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
  Western Blot: 1 - 3 µg/ml.
• Reactivities: Human, Monkey, Mouse, Bat, Equine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the N-terminus of human IRAK4 (NP_057207.2; NP_001107654.1)
• Specificity: This antibody reacts to the N-Terminus (aa2-13) of Interleukin 1 Receptor-Associated Kinase 4 (IRAK4).
• Formulation: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 1 receptor associated kinase 4
• Database Link:
  Entrez Gene 266632 MouseEntrez Gene 51135 Human
  UniProt ID Q9NWZ3
• Background: IRAK4, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK4 has auto- and cross-phosphorylation kinase activity and has been shown to phosphorylate and activate IRAK1. Additionally, IRAK4 interacts with IRAK1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK4 can activate NF-kappaB as well as mitogen-activated protein (MAP) kinase pathways. IRAK4 (-/-) mice are resistant to a lethal dose of lipopolysaccharide and are severely impaired in their responses to viral and bacterial challenges. At least two mRNA transcripts have been reported: 3.0- and 4.4-kb. IRAK4, also referrred to as NY-REN-64, was first identified as an antigen in a screen of renal tumors.
• Synonyms: IRAK-4, NY-REN-64

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway