SMAD1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP23424PU-N

SMAD1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot with Smad 1, 2, 3, 5 Polyclonal Antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1 μg/ml.
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human Smad 1,2,3,5 (442-456aa), identical to the related rat sequence
• Specificity: This antibody detects SMAD1/2/3/5 (C-term, 442-456). No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: SMAD family member 1
• Database Link:
  Entrez Gene 25671 RatEntrez Gene 4086 Human
  UniProt ID Q15797
• Background: SMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs,often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. Zhu, H et al concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-β superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.
• Synonyms: SMAD1,2,3,5, SMAD1, SMAD2, SMAD3, SMAD5

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway