CD18 (ITGB2) (N-term) Rabbit Polyclonal Antibody

CAT#: AP23400PU-N

CD18 (ITGB2) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

IHC staining with Integrin β2 Polyclonal Antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1-2 μg/ml.
  Immunohistochmistry on paraffin sections: 1-2 μg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide mapping at the N-terminal of human Integrin β2
• Specificity: This antibody detects CD18 / ITGB2 (N-term). No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit beta 2
• Database Link:
  Entrez Gene 16414 MouseEntrez Gene 309684 RatEntrez Gene 3689 Human
  UniProt ID P05107
• Background: The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The 3 alpha integrin chains associated individually with the beta-2 chain as a heterodimer have gene designations of ITGAL, ITGAM, and ITGAX, and leukocyte antigen designations of CD11A, CD11B, and CD11C, respectively. The expression of CD18 was increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 211. The ITGB2 gene spans approximately 40 kb and contains 16 exons and all exon/intron boundaries conform to the GT/AG splicing consensus. Furthermore,ITGB2 was constitutively clustered. Although it was expressed on the cell surface at normal levels and was capable of function following extracellular stimulation, it could not be activated via the “inside-out” signaling pathways.
• Synonyms: Integrin beta-2, MFI7

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Viral myocarditis