Sonic Hedgehog (SHH) (N-term) Rabbit Polyclonal Antibody

CAT#: AP23351PU-N

Sonic Hedgehog (SHH) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat brain tissue lysis using SHH antibody

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  Immunohistochemical analysis of paraffin embedded rat tissue sections (brain), staining SHH in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect SHH in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect SHH in formalin fixed and paraffin embedded tissues.
  Immunocytochemistry.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence near the N-terminal of human SHH
• Specificity: This antibody detects Sonic hedgehog (SHH) near N-term. No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: sonic hedgehog
• Database Link:
  Entrez Gene 20423 MouseEntrez Gene 29499 RatEntrez Gene 6469 Human
  UniProt ID Q15465
• Background: The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined; however, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.
• Synonyms: Sonic hedgehog protein, HHG-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer