CD40L (CD40LG) (N-term) Rabbit Polyclonal Antibody

CAT#: AP23309PU-N

CD40L (CD40LG) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat tissue sections (spleen) using CD40L antibody

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  Immunohistochemical analysis of paraffin embedded Mammary cancer sections staining CD40L in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1-2 μg/ml with the appropriate system to detect CD40L in cells and tissues.
  Immunohistochemistry on paraffin sections: 1-2 μg/ml to detect CD40L in formalin fixed and paraffin embedded tissues.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence at the N-terminal of human CD40L
• Specificity: This antibody detects CD154 / CD40L at N-term. No cross reactivity with other proteins.
• Formulation: 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD40 ligand
• Database Link:
  Entrez Gene 84349 RatEntrez Gene 959 Human
  UniProt ID P29965
• Background: CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
• Synonyms: CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Cytokine-cytokine receptor interaction, Primary immunodeficiency, Systemic lupus erythematosus, T cell receptor signaling pathway, Viral myocarditis