PTGS1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP23304PU-N

PTGS1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat testicle tissue lysis using COX1 antibody

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  AP23304PU-N COX1 antibody staining of Paraffin Embedded Rat Spleen Tissue Sections in cytoplasm. DAB chromogenic reaction.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: At 2μg/ml with the appropriate system to detect COX1 in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect COX1 in formalin fixed and paraffin embedded tissues.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence near the N-terminal of human PTGS1(COX1)
• Specificity: This antibody detects Cyclooxygenase 1 near N-term. No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: prostaglandin-endoperoxide synthase 1
• Database Link:
  Entrez Gene 19224 MouseEntrez Gene 24693 RatEntrez Gene 5742 Human
  UniProt ID P23219
• Background: Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1), is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD.By analysis of a human/hamster somatic hybrid DNA panel, Funk et al. (1991) demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.
• Synonyms: PGH synthase 1, PGHS-1, COX-1, COX1, PTGS1, Cyclooxygenase-1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways