ATP5PF Rabbit Polyclonal Antibody

CAT#: AP23301PU-N

ATP5PF rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat tissue sections (liver) lysis using ATP-5J antibody

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  Immunohistochemical analysis of paraffin embedded Mammary cancer sections, staining ATP-5J in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: At 0.5-1μg/ml with the appropriate system to detect ATP5J in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect ATP5J in formalin fixed and paraffin embedded tissues.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide mapping at the middle region of human mature ATP5J (CF6)
• Specificity: This antibody detects ATPase subunit F6 (mid region).
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6
• Database Link:
  Entrez Gene 11957 MouseEntrez Gene 94271 RatEntrez Gene 522 Human
  UniProt ID P18859
• Background: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 (ATP5J) is a multisubunit membrane-bound enzyme complex consisting of an F0 segment embedded in the membrane and an F1 segment attached to the F0. It is also a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. Human ATP5J shares 72% sequence identity with rat ATP5J.This import signal peptide is rich in basic amino acids, devoid of acidic amino acids, and amphiphilic, which allows it to be water-soluble yet capable of passage through the phospholipid membrane bilayers. Moreover, it is circulating and functions as an endogenous vasoconstrictor by inhibiting cytosolic phospholipase A2.
• Synonyms: ATP5J, ATP5A, ATPM, F1F0 ATPase subunit F6, Complex V subunit F6

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease