DLL4 Rabbit Polyclonal Antibody

CAT#: AP21750PU-N

DLL4 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Human sDLL-4 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sDLL-4 (AP21750BT-N or AP21750BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sDLL-4.
  Western Blot: To detect Human sDLL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsDLL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant Human sDLL-4.
• Specificity: Recognizes Human sDLL-4
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Purification: Affinity chromatography employing immobilized Human sDLL-4 matrix.
• Conjugation: Unconjugated
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: delta like canonical Notch ligand 4
• Database Link:
  Entrez Gene 54567 Human
  UniProt ID Q9NR61
• Background: DLL4 is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.
• Synonyms: Delta 4, Drosophila Delta homolog 4

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: Notch signaling pathway