Complement C5 (C5) Goat Polyclonal Antibody

CAT#: AP21462SU-N

Complement C5 (C5) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used in precipitating techniques as Immunoelectrophoresis and single or Double Radial Immunodiffusion to identify the presence of C5 containing complexes in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not
  exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.
  Recommended Dilutions:
  Immunoelectrophoresis: Use 2 µl human plasma or equivalent against 120 µl antiserum.
  Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  Antibody Titre: Precipitin titre 1/32 when tested against in agar-block immunodiffusion titration.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: C5 protein is isolated as a homogenous protein for use in antiserum production.
  Freund’s complete adjuvant is used in the first step of the immunization
• Specificity: The reactivity of the antiserum is restricted to C5.
  In immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum.
  The antiserum does not cross react with any other component of human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: State: Serum
  State: Lyophilized, Delipidated, Heat inactivated, Stable Whole antiserum without preservatives
• Reconstitution Method: Restore by adding 1 ml of sterile distilled water.
• Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal goat serum. No foreign proteins added.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.
• Stability: Shelf life: one year from despatch
• Gene Name: complement component 5
• Database Link:
  Entrez Gene 727 Human
  UniProt ID P01031
• Background: C5 component of human complement is a plasma protein consisting of two polypeptide chains, alpha, beta, bound by disulphide bonds. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack complex. The average concentration in plasma is about 70 µg/ml.
• Synonyms: CPAMD4, Complement component 5
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus