C3 Goat Polyclonal Antibody

CAT#: AP21439SU-N

C3 goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Immunoelectrophoresis: Use 2 µl Rat plasma or equivalent against 120 µl antiserum.
  Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  Single Radial Immunodiffusion: Use 1% antiserum in the gel.
• Reactivities: Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: C3c isolated and purified from pooled normal Rat serum by precipitation techniques, followed by Chromatographical methods.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: In Immunoelectrophoresis against fresh Rat serum, a single precipitin line is obtained in the beta-1 region representing native C3.
  Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain.
  The product does not react with any other protein components of Rat serum or plasma.
  Cross-reactivity:
  The antiserum does not cross-react with any other component of Rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
  Adsorption:
  Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
  Precipitin titre: 1/16 when tested against pooled normal rat serum in agar-block immunodiffusion titration.
• Formulation: State: Serum
  State: Lyophilized (Delipidated, heat inactivated) stable whole serum without preservatives.
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled goat serum. No foreign proteins added.
• Conjugation: Unconjugated
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Gene Name: complement component 3
• Database Link:
  Entrez Gene 24232 Rat
  UniProt ID P01026
• Background: C3 is the most abundant complement protein in rat serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways.
  Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle; degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response.
• Synonyms: CPAMD1, Complement component 3