AOR_1_212054 Rabbit Polyclonal Antibody

CAT#: AP21422BT-N

AOR_1_212054 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques
  Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/10-1/100.
• Reactivities: Aspergillus
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Ribonuclease T1 isolated and purified from Aspergillus oryzae.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized IgG fraction
  Molar radio: Biotin/IgG ~5.1
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00651
• Synonyms: Guanyl-specific ribonuclease T1, rntA
• Note: Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques.